Part:BBa_K1172505:Design

Outer membrane porin OprF from Pseudomonas fluorescens with medium Anderson promotor and medium RBS

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 826
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1218
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 739
  

Design Notes

• Classical [http://2013.igem.org/Team:Bielefeld-Germany/Labjournal/ProtocolsPrograms#Suffix_Insertion Suffix-Insertion] of the Biobrick BBa_K1172501 into the Backbone of the BioBrick BBa_K608006.

Source

Pseudomonas fluorescens Migula 1895 genomic DNA

DSMZ No.: 7355